Discover everything you need to know about freeze & thaw processes in our detailed articles. Learn about different methods, best practices, and advanced cryopreservation techniques to ensure the highest quality and stability of your biopharmaceutical products. Explore how our innovative freezing solutions are applied across various areas, including cell banking, monoclonal antibody preservation, and bioconjugate stability. Single Use Support offers state-of-the-art systems that protect sensitive products during temperature transitions, maintaining consistency and quality throughout.
Freeze & Thaw
Once biologics are produced, they need to be stored for shorter or longer periods of time, with dedicated storage requirements to be met. Here, we will uncover these requirements, along with solutions to fulfill them.
Alexander Fuchs
May 8, 2024
Freeze & Thaw
Prior to be used in diagnostics or therapeutics, proteins have to be safely stored. In this article, we will assess methods to preserve their stability upon storage.
Alexander Fuchs
April 10, 2024
Freeze & Thaw
Protein stability is crucial to be maintained along the manufacturing process of protein-based products. In this article, we will discover what influences protein stability, along with methods to assess and increase it.
Khalil Essani
April 9, 2024
Freeze & Thaw
Active pharmaceutical ingredients (APIs) are fundamental for drug products to be effective, but require special treatment – e.g. considering humidity, light, or temperature. In this article, we will highlight the different cold storage requirements of APIs, including examples and an overview of different practical approaches.
Alexander Fuchs
March 21, 2024
Freeze & Thaw
The objective of an optimal cryopreservation strategy is to deactivate (put on hold) degenerative cellular pathways and preserve proliferative potential by reducing the temperature below –130°C. To reduce the effects of ice crystal and osmotic injuries, cryopreservation protocols usually incorporate cryoprotective agents (CPAs).
Khalil Essani
February 8, 2024
Freeze & Thaw
Freezing small volumes extends beyond laboratory settings to include commercialized manufacturing processes for small batches in biomanufacturing, like cell banking, gene therapies utilizing viral vectors, lipid nanoparticles, and fill & finish procedures.
Alexander Fuchs
November 20, 2023
Freeze & Thaw
In the biopharmaceutical sector, the utilization of F-Gases is on a decline. However, the need for cryopreservation of drug substances will remain. In order to contribute to a more environmentally friendly freezing of drugs, more sustainable technologies need to be developed and applied. But is sustainable freezing even possible?
Alexander Fuchs
September 6, 2023
Freeze & Thaw
The preservation of cells at cryogenic temperature can be achieved using different techniques. This is far from a simple freezing process; it's a carefully controlled method that prevents the formation of ice crystals, thus maintaining the integrity of the biological material.
Brian Moloney
August 11, 2023
Freeze & Thaw
This guide provides an overview of the necessary steps and considerations for preparing cells for cryopreservation. Key topics include selecting appropriate cells, cultivating and harvesting them under controlled conditions, utilizing proper cryoprotective agents, and applying specific freezing techniques.
Brian Moloney
August 11, 2023
Freeze & Thaw
Cell thawing is a critical procedure in the area of life science and medicine. Ensuring a gentle thawing of frozen cells is essential for preserving their viability and functionality. In this article, we will highlight the cell thawing process, discuss common challenges, and provide recommendations for achieving optimal results in cell banking.
Brian Moloney
August 9, 2023
Freeze & Thaw
It is many decades since cryovessels emerged as a groundbreaking solution in the biopharmaceutical industry, enabling the safe and efficient storage of bulk drug substances. However, the introduction of plate-based freezing methods has further advanced the technology available for the freezing of biopharmaceuticals, offering improved product quality and consistency.
Daniel Tischler
July 28, 2023
Freeze & Thaw
Not all biopharmaceuticals are suitable for freeze-drying. Depending on the specific stability requirements the preservation of biopharmaceuticals is either with cryopreservation or lyophilization.
Michael Eder
July 28, 2023
Articles by Application Area:
Articles by Solution:
E-Book
The process of freezing can lead to a denaturation of the protein. In fact, parts of the protein solution will always show less activity based on the stress the protein is exposed to during freezing and thawing. It is therefore important to optimize the freezing and thawing process in order to minimize activity loss. A key parameter for this process is the freezing or thawing rate.
E-Book
Scalability around freezing means the ability to guarantee constant freezing kinetics on proteins (biopharmaceutical molecules) in all scales, filling volumes and loading scenarios. This guide covers how to achieve scalable freezing & thawing of biopharmaceuticals by showing What is scalability? Setup for controlled icefront growth speed Scalability results Outcomes
E-Book
The study was conducted aiming to optimize the cryogenic freezing process of the mammalian CHO-K1 cell line with an innovative controlled rate liquid nitrogen freezer (RoSS.LN2F). The study incorporated three viability assays to comprehensively assess the impact of varying freezing rates on post-thaw cell recovery | Trypan blue | LIVE/DEAD | Fluorescence.